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Mary Jackson

Associate Professor
Director, Mycobacteria Research Laboratories​

Lab Location: B426 Microbiology Building
Office: B423A Microbiology Building
Phone: (970) 491-3582

Research Interests and Accomplishments

Our research focuses on the biosynthetic pathways (enzymes and transporters) of major cell envelope components - (lipo)polysaccharides, (glyc​o)lipids, and fatty acids - playing important roles in the physiology and/or pathogenicity of Mycobacterium tuberculosis and other paramount mycobacterial pathogens.

Another aspect of our research concerns the development of novel anti-mycobacterial drugs and the study of the mechanisms of resistance to biocides of slow- and fast-growing mycobacteria of clinical interest.

Finally, we are also interested in neglected and emerging non-tuberculous mycobacterial pathogens, including Mycobacterium leprae (the etiological agent of leprosy) and Mycobacterium abscessus complex species, and their strategies to persist in the environment, transmit to new hosts and cause disease.​

Research Highlights

Highlights from our work include the discovery of a number of enzymes (glycosyltransferases, acyltransferases, methyltransferases, polyketide synthases) and regulators involved in the biogenesis of mycobacterial glycoconjugates and the recognition of the pivotal role played by glycosyltransferases of the polyprenyl-phosphate-sugar-dependent class in particular in the building of the major cell envelope glycolipids and (lipo)polysaccharides, phosphatidylinositol mannosides, arabinogalactan and lipoarabinomannan. Another highlight was the discovery of a lipoprotein transporter (LppX) involved in the translocation of virulence-associated lipids (the phthiocerol dimycocerosates) to the cell surface of M. tuberculosis and the first demonstration that the translocation of complex lipids in mycobacteria involved mechanisms similar to those used by Gram-negative bacteria to export lipoproteins to the outer membrane.

In recent years, we elucidated how two former anti-TB drugs known as Isoxyl and Thiacetazone exert their bactericidal activity against M. tuberculosis and identified MmpL3 as an integral membrane transporter required for the export of hallmark entities of the cell envelope of all mycobacteria: the mycolic acids. The transport activity of MmpL3 is inhibited by several classes of anti-TB inhibitors currently under development.

Our recent studies on the environmental persistence of M. leprae led to the discovery that the leprosy bacillus may be kept viable and virulent for extended periods of time (> 8 months) outside a mammalian host, inside free-living amoebae.​

The Lab 

Undergraduate Students

  • Madison Nesiba
  • Tatyana A. Lyakhova

Graduate Students

  • Winona Burgess (combined DVM residency/MS program)
  • Crystal Elenbaas (PhD program)
  • David Markman (MS program)

Post-doctoral Fellows

  •  Zuzana Palceková

Research Scientists

  • Anna Grzegorzewicz
  • Shiva kumar Angala
  • Juan Manuel Belardinelli
  • Wei Li

Research Associates

  • Vicki Jones  (Lab Manager)
  • Vinicius Calado Nogueira de Moura

Publications (2007-2016)

Li, W., Obregón-Henao, A., Wallach, J. B., North, E. J., Lee, R. E., Gonzalez-Juarrero, M., Schnappinger, D., and Jackson, M. (2016) Therapeutic potential of the Mycobacterium tuberculosis mycolic acid transporter, MmpL3. Antimicrob. Agents Chemother. pii: AAC.00826-16 (in press)

Thanna, S., Knudson, S. E., Grzegorzewicz, A. E., Kapil, S., Goins, C. M., Ronning, D. R., Jackson, M., Slayden, R. A., and Sucheck, S. J. (2016) Synthesis and microbiological evaluation of 2-amino-4,5,6,7-tetrahydrothieno[2,3-c]pyridines against sensitive and drug resistant Mycobacterium tuberculosis. Org. Biomol. Chem. (in press)

Santangelo, M. P., Heuberger, A., Blanco, F., Forrellad, M., Taibo, C., Klepp, L., Sabio García, J., Nikel, P., Jackson, M., and Bigi, F. (2016) Metabolic profile of Mycobacterium smegmatis reveals Mce4 proteins are relevant for cell wall lipid homeostasis. Metabolomics (in press)

Angala, S. K., McNeil, M. R., Zou, L., Liav, A., Zhang, J., Lowary, T. L., and Jackson, M. (2016) Identification of a novel mycobacterial arabinosyltransferase activity which adds an arabinosyl residue to α-D-mannosyl residues. ACS Chem. Biol. (in press) doi:10.1021/acschembio.6b00093

Albesa-Jové D., Svetliková Z., Tersa M., Sancho-Vaello E., Carreras-Gonzalez A., Bonnet P., Arrasate P., Eguskiza A., Angala S. K., Cifuente J. O., Korduláková J, Jackson M., Mikusová K, Guerin M. E. (2016) Structural basis for selective recognition of acyl chains by the membrane-associated acyltransferase PatA. Nat. Commun. 11, 10906. doi: 10.1038/ncomms10906.

Larrouy-Maumus, G., Gilleron, M., Škovierová, H., Zuberogoitia, S., Brennan, P. J., Puzo, G., Jackson, M., and Nigou, J. (2015) A glycomic approach reveals a new mycobacterial polysaccharide. Glycobiology, 25, 1163-1171.

Upadhyay, A. Fontes, F., Gonzales-Juarrero, M., McNeil, M., Crans, D. Jackson, M. and Crick, D. (2015) Partial Saturation of Menaquinone in Mycobacterium tuberculosis:  Function and Essentiality of a Novel Reductase, MenJ. ACS Cent. Sci., 1, 292-302.

Hasan, N. A., Davidson, R. M., de Moura, V. C., Garcia, B., Reynolds, P. R., Epperson, L. E., Farias-Hesson, E., DeGroote, M.-A., Jackson, M., and Strong, M. (2015) Draft Genome Sequence of Mycobacterium chelonae Type Strain ATCC 35752. Genome Announc. 3, doi : 10.1128/genomeA.00536-15.

Wang, Q., Zhu, L., Jones, V., Wang, C., Hua, Y., Shi, X., Feng, X., Jackson, M., Niu, C., and Gao, Q. (2015) CpsA, a LytR-CpsA-Psr Family Protein in Mycobacterium marinum, is Required for Cell Wall Integrity and Virulence. Infect. Immun. 83, 2844-2854.

Wheat, W. H., Dhouib, R., Angala, S. K., Larrouy-Maumus, G., Dobos, K., Nigou, J., Spencer, J. S., and Jackson, M. (2015) The presence of a galactosamine substituent on the arabinogalactan of Mycobacterium tuberculosis abrogates full maturation of human peripheral blood monocyte-derived dendritic cells and increases secretion of IL-10 Tuberculosis (Edinb) 95, 476-489.

Grzegorzewicz, A. E., Eynard, N., Quémard, A., North, E. J., Margolis, A., Lindenberger, J. J., Jones, V., Korduláková, J., Brennan, P. J., Lee, R. E., Ronning, D. R., McNeil, M. R., and Jackson, M. (2015) Covalent modification of the Mycobacterium tuberculosis FAS-II dehydratase by Isoxyl and Thiacetazone. ACS Infect. Dis. 1, 91-97.

Jackson, M. (2014) The Mycobacterial Cell Envelope-Lipids. Cold Spring Harb. Perspect. Med. 4, a021105.

Daffé M., Crick D.C., and M. Jackson (2014). Genetics of capsular polysaccharides and cell envelope (glyco)lipids. Microbiol Spectrum 2(4):MGM2-0021-2013. doi:10.1128/microbiolspec.MGM2-0021-2013.

Wheat, W. H., Casali, A. L., Thomas, V., Spencer, J. S., Lahiri, R., Williams, D. L., McDonnell, G. E., Gonzalez-Juarrero, M., Brennan, P. J., and Jackson, M. (2014) Long-term survival and virulence of Mycobacterium leprae in amoebal cysts. PLoS Negl. Trop. Dis. 8(12):e3405. doi: 10.1371/journal.pntd.0003405.

Angala, S. K.; Belardinelli, J. M.; Huc-Claustre, E.; Wheat, W. H.; Jackson, M. (2014) The cell envelope glycoconjugates of Mycobacterium tuberculosis. Crit. Rev. Biochem. Mol. Biol. 49 (5), 361-399.

North, E. J., Jackson, M., and Lee, R. E. (2014) New Approaches to Target the Mycolic Acid Biosynthesis Pathway for the Development of Tuberculosis Therapeutics. Curr. Pharm. Des. 20, 4357-4378.

Albesa-Jové D., Giganti D., Jackson M., Alzari P. M., Guerin M. E. (2014). Structure-function relationships of membrane-associated GT-B glycosyltransferases. Glycobiology 24: 108-124.

Shukla, S., Richardson, E. T., Athman, J. J., Shi, L., Wearsch, P. A., McDonald, D., Banaei, N., Boom, W. H., Jackson, M., and Harding, C. V. (2014) Mycobacterium tuberculosis lipoprotein LprG binds lipoarabinomannan and determines its cell envelope localization to control phagolysosomal fusion. PLoS Pathog 10, e1004471

Kaur, D., Angala, S. K., Wu, S.-W., Khoo, K.-H., Chatterjee, D., Brennan, P. J., Jackson, M.*, and McNeil*, M. R. (2014) A single arabinan chain is attached to the phosphatidylinositol mannosyl core of the major immunomodulatory mycobacterial cell envelope glycoconjugate, lipoarabinomannan. J. Biol. Chem. 289, 30249-30256  (*co-senior authors)

Belardinelli, J. M., Larrouy-Maumus, G., Jones, V., de Carvalho, L. P., McNeil, M. R., and Jackson, M. (2014) Biosynthesis and translocation of unsulfated acyltrehaloses in Mycobacterium tuberculosis. J. Biol. Chem. 289, 27952-27965.

Gaur, R. L., Ren, K., Blumenthal, A., Bhamidi, S., Gibbs, S., Jackson, M., Zare, R. N., Ehrt, S., Ernst, J. D., and Banaei, N. (2014) LprG-mediated surface expression of lipoarabinomannan is essential for virulence of Mycobacterium tuberculosis. PLoS Pathog 10, e1004376.

Li, W., Upadhyay, A., Fontes, F. L., North, E. J., Wang, Y., Crans, D. C., Grzegorzewicz, A. E., Jones, V., Franzblau, S. G., Lee, R. E., Crick, D. C., and Jackson, M. (2014) Novel insights into the mechanism of inhibition of MmpL3, a target of multiple pharmacophores in Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 58, 6413-6423

De Groote, M. A., Gibbs, S., de Moura, V. C., Burgess, W., Richardson, K., Kasperbauer, S., Madinger, N., and Jackson, M. (2014) Analysis of a panel of rapidly growing mycobacteria for resistance to aldehyde-based disinfectants. Am. J. Infect. Control 42, 932-934

Yang, L., Lu, S., Belardinelli, J., Huc-Claustre, E., Jones, V., Jackson, M., and Zgurskaya, H. I. (2014) RND transporters protect Corynebacterium glutamicum from antibiotics by assembling the outer membrane. MicrobiologyOpen 3, 484-496

Svetlíková, Z., Barath, P., Jackson, M., Korduláková, J., and Mikušová, K. (2014) Purification and characterization of the acyltransferase involved in biosynthesis of the major mycobacterial cell envelope glycolipid - monoacylated phosphatidylinositol dimannoside. Protein Expr Purif 100, 33-39

Puckett, S., Trujillo, C., Eoh, H., Marrero, J., Spencer, J., Jackson, M., Schnappinger, D., Rhee, K., and Ehrt, S. (2014) Inactivation of fructose-1,6-bisphosphate aldolase prevents optimal co-catabolism of glycolytic and gluconeogenic carbon substrates in Mycobacterium tuberculosis. PLoS Pathog 10, e1004144.

de Moura, V. C., Gibbs, S., and Jackson, M. (2014) Gene replacement in Mycobacterium chelonae: application to the construction of porin knock-out mutants. PLoS ONE 9, e94951.

Capodagli, G. C., Sedhom, W. G., Jackson, M., Ahrendt, K. A. & Pegan, S. D. (2014) A noncompetitive inhibitor for Mycobacterium tuberculosis's class IIa fructose 1,6-bisphosphate aldolase. Biochemistry 53 (1), 202-213.

Forrellad, M. A., McNeil, M., Santangelo, M. P., Blanco, F. C., García, E., Repett, N., Klepp, L. I., Huff, J., Niederweis, M., Jackson, M. & Bigi, F. (2014). Role of the Mce1 transporter in the lipid homeostasis of Mycobacterium tuberculosis. Tuberculosis (Edinb) 94, 170-177.

Tettelin, H., Davidson, R. M., Agrawal, S., Aitken, M. L., Shallom, S., Hasan, N. A., Strong, M., Nogueira de Moura, V. C., De Groote, M. A., Duarte, R. S., Hine, E., Parankush, S., Su, Q., Daugherty, S. C., Fraser, C. M., Brown-Elliott, B. A., Wallace, R. J., Jr., Holland, S. M., Sampaio, E. P., Olivier, K. N., Jackson, M. & Zelazny, A. M. (2014). High-level Relatedness among Mycobacterium abscessus subsp. massiliense Strains from Widely Separated Outbreaks. Emerg Infect Dis 20, 364-371.

Favrot L., Grzegorzewicz A. E., Lajiness D. H., Marvin R. K., Boucau J., Isailovic D., Jackson M., Ronning D. R. (2013). Mechanism of inhibition of Mycobacterium tuberculosis antigen 85 by ebselen. Nat Commun 4: 2748.

Grzegorzewicz, A. E., and M. Jackson. (2013). Subfractionation and Analysis of the Cell Envelope (Lipo)polysaccharides of Mycobacterium tuberculosis. Methods Mol. Biol. 966: 309-324.

Deshayes, C., Angala, S. K., Marion, E., Brandli, I., Babonneau, J., Preisser, L., Eyangoh, S., Delneste, Y., Legras, P., De Chastellier, C., Stinear, T. P., Jackson, M. & Marsollier, L. (2013). Regulation of Mycolactone, the Mycobacterium ulcerans Toxin, Depends on Nutrient Source. PLoS Negl Trop Dis 7, e2502.

Davidson, R. M., Hasan, N. A., Calado Nogueira de Moura, V., Duarte, R. S., Jackson, M., Strong, M. (2013) Phylogenomics of Brazilian epidemic isolates of Mycobacterium abscessus subsp bolletii reveals relationships of global outbreak strains. Infect. Genet. Evol. 20C :292-297.

Davidson R. M., Reynolds P. R., Farias-Hesson E., Duarte R. S., Jackson M., Strong M. (2013) Genome sequence of an outbreak strain of Mycobacterium abscessus subsp bolletii from Rio de Janeiro, Brazil. Genome Announcements 1(4): doi:pii: e00617-13. 10.1128/genomeA.00617-13.

Pethe K., Jang J., Kang S., Park S., Ahn S., Jiricek J., Jung J., Jeon H. K., Cechetto J., Christophe T., Kempf M., Jackson M., Lenaerts A., Pham H., Jones V., Seo M. J., Lee S., Kim Y. M., Seo M., Seo J. J., Ko Y., Choi I., Kim R., Kim S., Lim S. B., Yim S. A., Nam J., Kang H., Oh C.-T., Cho Y., Jang Y., Cho Y., Kim J., Chua A., Tan B. H., Nanjundappa M. B., Rao S. P. S., Barnes W. S., Wintjens R., Walker J. R., Alonso S., Kim J., Oh S., Han S.-J., No Z., Lee J., Brodin P., Nam K., Bifani P., Kim J. (2013). Discovery of Q203, a potent clinical candidate for the treatment of Tuberculosis. Nat. Med. 19:1157-1160. 

Jackson, M., M. R. McNeil, and P. J. Brennan. (2013). Progress in targeting cell envelope biogenesis in Mycobacterium tuberculosis. Future Microbiol. 8(7):855-875. (review article)

Liu, C. F., L. Tonini, W. Malaga, M. Beau, A. Stella, D. Bouyssié, M. Jackson, J. Nigou, G. Puzo, C. Guilhot, O. Burlet-Schiltz, and M. Rivière. 2013. Bacterial protein O-mannosylating enzyme is crucial for virulence of Mycobacterium tuberculosis. Proc. Natl. Acad. Sci. USA 110:6560-6565.

Ovrutsky, A. R., E. D. Chan, M. Kartalija, X. Bai, M. Jackson, S. Gibbs, J. O. Falkinham III, M. D. Iseman, P. R. Reynolds, G. McDonnell, and V. Thomas. 2013. Co-occurrence of free-living amoebae and non-tuberculous mycobacteria in hospital water networks, and preferential growth of Mycobacterium avium in Acanthamoeba lenticulata. Appl. Environ. Microbiol. 79:3185-3192.

North, E. J., M. S. Scherman, D. F. Bruhn, J. S. Scarborough, M. M. Maddox, V. Jones, A. Grzegorzewicz, L. Yang, T. Hess, C. Morisseau, M. Jackson, M. R. McNeil, and R. E. Lee. (2013). Design, synthesis and anti-tuberculosis activity of 1-adamantyl-3-heteroaryl ureas with improved in vitro pharmacokinetic properties. Bioorg. Med. Chem. 21:2587-2599. 

Slayden, R. A., M. Jackson, J. Zucker, M. V. Ramirez, C. C. Dawson, R. Crew, N. S. Sampson, S. T. Thomas, N. Jamshidi, P. Sisk, R. Caspi, D. C. Crick, M. R. McNeil, M. S. Pavelka, M. Niederweis, A. Siroy, V. Dona, J. McFadden, H. Boshoff, and J. M. Lew. (2013). Updating and curating metabolic pathways of TB. Tuberculosis (Edinb) 93:47-59.

Spencer, J. S., M. S. Duthie, A. Geluk, M. F. Balagon, H. J. Kim, W. H. Wheat, D. Chatterjee, M. Jackson, WLi, J. N. Kurihara, A. Maghanoy, I. Mallari, P. Saunderson, P. J. Brennan, and H. M. Dockrell. (2012). Identification of Serological Biomarkers of Infection, Disease Progression and Treatment Efficacy for Leprosy. Mem. Inst. Oswaldo Cruz. 107(Suppl 1) : 79-89.

Larrouy-Maumus, G., Škovierová, H., Dhouib, R., Angala, S. K., Zuberogoitia, S., Pham, H., Drumond Villela, A., Mikušová, K., Noguera, A., Gilleron, M., Valentinova, L., Korduláková, J., Brennan, P. J., Puzo, G., Nigou, J., and M. Jackson (2012) A small multidrug resistance-like transporter involved in the arabinosylation of arabinogalactan and lipoarabinomannan in mycobacteria. J. Biol. Chem. 287(47): 39933-39941.

Grzegorzewicz, A. E., J. Korduláková, V. Jones, S. E. M. Born, J. M. Belardinelli, A. Vaquié, V. A. K. B. Gundi, J. Madacki, N. Slama, F. Laval, J. Vaubourgeix, R. M. Crew, B. Gicquel, M. Daffé, H. R. Morbidoni, P. J. Brennan, A. Quémard, M. R. McNeil, and M. Jackson. (2012). A common mechanism of inhibition of the Mycobacterium tuberculosis mycolic acid biosynthetic pathway by Isoxyl and Thiacetazone. J. Biol. Chem. 287(46): 38434-38441. 

Urresti, S., D. Albesa-Jove, F. Schaeffer, H. T. Pham, D. Kaur, P. Gest, M. J. van der Woerd, A. Carreras Gonzalez, S. Lopez Fernandez, P. M. Alzari, P. J. Brennan, M. Jackson*, and M. E. Guerin* (2012). Mechanistic insights into the retaining glucosyl-3-phosphoglycerate synthase from mycobacteria. J. Biol. Chem. 287(29):24649-24661.  (*co-senior authors) 

Scherman, M. S., E. J. North, V. Jones, T. N. Hess, A. E. Grzegorzewicz, T. Kasagami, I.-H. Kim, O. Merzlikin, A. J. Lenaerts, R. E. Lee, M. Jackson, C. Morisseau, and M. R. McNeil (2012). Screening a library of 1,600 adamantyl ureas for anti-Mycobacterium tuberculosis activity in vitro and for better physical chemical properties for bioavailability. Bioorg. Med. Chem. 20(10): 3255-3262.

Klepp, L. I., M. A. Forrellad, A. V. Osella, F. Blanco, E. J. Stella, M. V. Bianco, M. P. Santangelo, C. Sassetti, M. Jackson, A. A. Cataldi, F. Bigi, and H. R. Morbidoni (2012). Impact of the deletion of the six mce operons in Mycobacterium smegmatis. Microbes and Infection 14(7-8) :590-599.

Grzegorzewicz, A. E., H. Pham, V. A. K. B. Gundi, M. S. Scherman, E. J. North, T. Hess, V. Jones, V. Gruppo, S. E. M. Born, J. Korduláková, S. S. Chavadi, C. Morisseau, A. J. Lenaerts, R. E. Lee, M. R. McNeil, and M. Jackson (2012) Inhibition of mycolic acid transport across the Mycobacterium tuberculosis plasma membrane. Nat. Chem. Biol. 8(4):334-341.

Fisher, C. W., A. Fiorello, D. Shaffer, M. Jackson, and G. E. McDonnell (2012). Aldehyde-resistant mycobacteria associated with the use of endoscope reprocessing systems. Am. J. Infect. Control  40(9):880-882. 

Santangelo, M. P., P. M. Gest, M. E. Guerin, M. Coinçon, H. Pham, G. Ryan, S. E. Puckett, J. S. Spencer, M. Gonzalez-Juarrero, R. Daher, A. J. Lenaerts, D. Schnappinger, M. Therisod, S. Ehrt, J. Sygusch, and M. Jackson (2011). Glycolytic and non-glycolytic functions of the fructose-1,6-bisphosphate aldolase of Mycobacterium tuberculosis, an essential enzyme produced by replicating and non-replicating bacilli. J. Biol. Chem. 286(46): 40219-40231. 

Dianišková, P., J. Korduláková, H. Škovierová, D. Kaur, M. Jackson, P. J. Brennan, and K. Mikušová (2011). Investigation of ABC transporter from mycobacterial arabinogalactan biosynthetic cluster. Gen. Physiol. Biophys. 30:239-250. 

Shang, S., S. Gibbs, M. Henao-Tamayo, C. A. Shanley, G. McDonnell, R. S. Duarte, D. J. Ordway, and M. Jackson (2011). Increased virulence of an epidemic strain of Mycobacterium massiliense in mice. PLoS ONE  6(9): e24726.

Brown, J. R., E. J. North, J. G. Hurdle, C. Morisseau, J. S. Scarborough, D. Sun, J. Korduláková, M. S. Scherman, V. Jones, A. Grzegorzewicz, R. M. Crew, M. Jackson, M. R. McNeil, and R. E. Lee (2011). The structure-activity relationship of urea derivatives as anti-tuberculosis agents. Bioorg Med Chem 19(18): 5585-5595. 

Zhang, J., S. K. Angala, P. K. Pramanik, K. Li, D. C. Crick, A. Liav, A. Jozwiak, E. Swiezewska, M. Jackson, and D. Chatterjee (2011). Reconstitution of functional mycobacterial arabinosyltransferase AtfC proteoliposome and assessment of decaprenylphosphorylarabinose analogues as arabinofuranosyl donors. ACS Chem. Biol. 6: 819-828.

Jackson, M., and Yamamoto, S. (2011). Chapter 1: Historical background of Mycobacterium bovis BCG, p. 3-12. In T. Takii, J. Maeyama, and S. Yamamoto (ed.), BCG − Vaccine and Adjuvant. Japan Anti-Tuberculosis Association, Tokyo, Japan.

Škovierová, H., G. Larrouy-Maumus, H. Pham, M. Belanová, N. Barilone, A. DasGupta, K. Mikušová, B. Gicquel, M. Gilleron, P. J. Brennan, G. Puzo, J. Nigou, and M. Jackson (2010). Biosynthetic origin of the galactosamine substituent of arabinogalactan in Mycobacterium tuberculosis. J. Biol. Chem. 285(53): 41348-41355. 

Termentzi, A., Khouri, I., Gaslonde, T., Prado, S., Saint-Joanis, B., Bardou, F., Amanatiadou, E. P., Vizirianakis, I. S., Korduláková, J., Jackson, M., Brosch, R., Janin, Y. L., Daffé, M., Tillequin, F., and Michel, S. (2010) Synthesis, biological activity, and evaluation of the mode of action of novel antitubercular benzofurobenzopyrans substituted on A ring. Eur. J. Med. Chem. 45(12): 5833-5847.

Daher, R., M. Coinçon, M. Fonvielle, P. Gest, M. Guerin, M. Jackson, J. Sygusch, and M. Thérisod (2010). Rational design, synthesis and evaluation of new selective inhibitors of microbial class II (zinc dependent) fructose bis-phosphate aldolases. J. Med. Chem. 53: 7836-7842.

Guerin, M. E., J. Korduláková, P. M. Alzari, P. J. Brennan, and Jackson, M. (2010). Molecular basis of phosphatidylinositol mannoside biosynthesis and regulation in mycobacteria. J. Biol. Chem. 285: 33577-33583. 

Dasgupta, A., K. Sureka, D. Mitra, B. Saha, S. Sanyal, A. K. Das, P. Chakrabarti, M. Jackson, B. Gicquel, M. Kundu, and J. Basu (2010). An oligopeptide transporter of Mycobacterium tuberculosis regulates cytokine release and apoptosis of infected macrophages. PLoS ONE 5(8):e12225. 

Christophe, T., M. Jackson, H. K. Jeon, D. Fenistein, M. Contreras Dominguez, J. Kim, A. Genovesio, J.-P. Carralot, F. Ewann, E. H. Kim, S. Y. Lee, S. Kang, M. J. Seo, E. J. Park, H. Škovierová, H. Pham, G. Riccardi, J. Y. Nam, L. Marsollier, M. Kempf, M.-L. Joly-Guillou, T. Oh, W. K. Shin, Z. No, U. Nehrbass, R. Brosch, S. T. Cole, and P. Brodin. (2009). High content screening identifies decaprenyl-phosphoribose 2' epimerase as a target for intracellular antimycobacterial inhibitors. PLoS Pathog. 5(10):e10000645. doi:10.1371/journal.ppat.1000645.

Scherman, H., D. Kaur, H. Pham, H. Škovierová, M. Jackson, and P. J. Brennan. (2009) Identification of a polyprenylphosphomannosyl synthase involved in the synthesis of mycobacterial mannosides. J. Bacteriol. 191: 6769-6772.

Kaur, D., Guerin, M. E., Škovierová, H., Brennan, P. J. and Jackson, M. (2009). Chapter 2. Biogenesis of the cell wall and other glycoconjugates of Mycobacterium tuberculosis. Advances in Applied Microbiology. 69: 23-78. (review article)

Guerin, M. E., D. Kaur, B. S. Somashekar, S. Gibbs, P. Gest, D. Chatterjee, P. J. Brennan, and Jackson, M. (2009) New insights into the early steps of phosphatidylinositol mannosides biosynthesis in mycobacteria. PimB' is an essential enzyme of Mycobacterium smegmatis. J. Biol. Chem. 284: 25687-25696.

Škovierová, H., G. Larrouy-Maumus, J. Zhang, D. Kaur, N. Barilone, J. Korduláková, M. Gilleron, S. Guadagnini, M. Belanova, M.-C. Prevost, B. Gicquel, G. Puzo, D. Chatterjee, P. J. Brennan, J. Nigou, and Jackson, M. (2009) AftD, a novel essential arabinofuranosyltransferase from mycobacteria. Glycobiology 19: 1235-1247.

Svetlíková, Z., H. Škovierová, M. Niederweis, J.-L. Gaillard, G. McDonnell and Jackson, M. (2009) The role of porins in the susceptibility of Mycobacterium smegmatis and Mycobacterium chelonae to aldehyde-based disinfectants and drugs. Antimicrob Agents Chemother 53: 4015-4018.

Jackson, M., and Brennan, P.J. (2009) Polymethylated polysaccharides from Mycobacterium species revisited. J. Biol. Chem. 284:1949-1953.

Guerin, M. E., Schaeffer, F., Chaffotte, A., Gest, P., Giganti, D., Korduláková, J., van der Woerd, M., Jackson, M. and Alzari, P. M. (2009). Substrate-induced conformational changes in the essential peripheral membrane-associated mannosyltransferase PimA from Mycobacteria. Implications for catalysis. J Biol Chem 284: 21613-21625.

Santangelo, M. P., L. Klepp, J. Nuñez-García, F. Blanco, M. Soria, M. del Carmen García-Pelayo, M. V. Bianco, A. Cataldi, P. Golby, M. Jackson, S. V. Gordon and F. Bigi. (2009) Mce3R, a TetR-type transcriptional repressor, controls the expression of a regulon involved in lipid metabolism in Mycobacterium tuberculosis. Microbiology. 155 : 2245-2255.

Kaur, D., Pham, H., Larrouy-Maumus, G., Rivière, M., Vissa, V., Guerin, M. E., Puzo, G., Brennan, P. J. and Jackson, M. (2009). Initiation of methylglucose lipopolysaccharide biosynthesis in mycobacteria. PLoS ONE. 4(5): e5447. doi:10.1371/journal.pone.0005447.

Gest, P., Kaur, D., Pham, H.T., van der Woerd, M., Hansen, E., Brennan, P.J., Jackson, M., and Guerin, M.E. (2008) Preliminary crystallographic analysis of GpgS, a key glucosyltransferase involved in methylglucose lipopolysaccharide biosynthesis in Mycobacterium tuberculosis. Acta Crystallogr Sect F Struct Biol Cryst Commun F64: 1121–1124.

Kaur, D., Obregón-Henao, A., Pham, H., Chatterjee, D., Brennan, P.J., and Jackson, M. (2008) Lipoarabinomannan of Mycobacterium; mannose capping by a multifunctional terminal mannosyltransferase. Proc. Natl. Acad. Sci. USA 105: 17973-17977.

Gilleron, M., Jackson, M., Nigou, J., and Puzo, G. (2008) Structure, activities and biosynthesis of the Phosphatidyl-myo-Inositol-based lipoglycans In The Mycobacterial Cell Envelope: an overview. Editors: M. Daffé & J.-M. Reyrat; ASM Press, Washington DC. Chap. 6, 75-105. (review)

Sambou, T., Dinadayala, P., Stadthagen, G., Barilone, N., Bordat, Y., Constant, P., Levillain, F., Neyrolles, O., Gicquel, B., Lemassu, A., Daffé, M., and Jackson, M. (2008) Capsular glucan and intracellular glycogen of Mycobacterium tuberculosis: Biosynthesis and impact on the persistence in mice. Mol. Microbiol. 70: 762-774.

Fonvielle, M., Coinçon, M., Daher, R., Desbenoit, N., Kosieradzka, K., Barilone, N., Gicquel, B., Sygusch, J., Jackson, M., and Therisod, M. (2008) Synthesis and biochemical evaluation of selective inhibitors of class II fructose bis-phosphate aldolases: towards new synthetic antibiotics. Chem. Eur. J. 14: 8521-8529.

Liav, A., Angala, S.K., Brennan, P.J., and Jackson, M. (2008) N-D-Aldopentofuranosyl-N’-[p-(isoamyloxy)phenyl]-thiourea derivatives: Potential anti-TB therapeutic agents. Bioorg. Med. Chem. Lett. 18: 2649-2651.

Pitarque, S., Larrouy-Maumus, G., Payré, B., Jackson, M., Puzo, G., and Nigou, J. (2008) The immunomodulatory lipoglycans, lipoarabinomannan and lipomannan, are exposed at the mycobacterial cell surface. Tuberculosis 88, pp. 560-565.

Chesne-Seck, M.-L., N. Barilone, F. Boudou, J. Gonzalo Asensio, P. E. Kolattukudy, C. Martin, S. T. Cole, B. Gicquel, D. N. Gopaul, and Jackson, M. (2008). A point mutation in the two-component regulator PhoP-PhoR accounts for the absence of polyketide-derived acyltrehaloses but not that of phthiocerol dimycocerosates in Mycobacterium tuberculosis H37Ra J. Bact. 190, pp. 1329-1334.

Berg, S., D. Kaur, M. Jackson, and P. J. Brennan. (2007) The glycosyltransferases of Mycobacterium tuberculosis- roles in the synthesis of arabinogalactan, lipoarabinomannan, and other glycoconjugates. Glycobiology 17:35R-56R.  (review article)

Jackson, M., Stadthagen, G., and Gicquel, B. (2007) Long-chain multiple methyl-branched fatty acid-containing lipids of Mycobacterium tuberculosis: biosynthesis, transport, regulation and biological activities. Tuberculosis 87 : 78-86.  (review article)

Korduláková, J., Y. L. Janin, A. Liav, N. Barilone, T. Dos Vultos, J. Rauzier, P. J. Brennan, B. Gicquel, and M. Jackson. (2007) Isoxyl activation by EthA is required for bacteriostatic activity against Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 51:3824-3829.

Kaur, D., M. R. McNeil, K.-H. Khoo, D. Chatterjee, D. C. Crick, M. Jackson, and P. J. Brennan. (2007) New insights into the biosynthesis of mycobacterial lipomannan arising from deletion of a conserved gene. J. Biol. Chem 282:27133-27140.

Stadthagen, G., Sambou, T., Guerin, M., Barilone, N., Boudou, F., Korduláková, J., Charles, P., Alzari, P.M., Lemassu, A., Daffé, M., Puzo, G., Gicquel, B., Rivière, M., and Jackson, M. (2007) Genetic basis for the biosynthesis of methylglucose lipopolysaccharides in Mycobacterium tuberculosis. J. Biol. Chem. 282: 27270-27276.

Guerin, M.E., Korduláková, J., Schaeffer, F., Svetlikova, Z., Buschiazzo, A., Giganti, D., Gicquel, B., Mikusová, K., Jackson, M., and Alzari, P.M. (2007) Molecular recognition and interfacial catalysis by the essential phosphatidylinositol mannosyltransferase PimA from mycobacteria. J. Biol. Chem 282: 20705-20714.

Marsollier, L., P. Brodin, M. Jackson, J. Korduláková, P. Tafelmeyer, E. Carbonnelle, J. Aubry, G. Milon, P. Legras, J.-P. Saint-Andre, C. Leroy, J. Cottin, M.-L. Joly Guillou, G. Reysset, and S. T. Cole. (2007) Impact of Mycobacterium ulcerans biofilm on tranmissibility to ecological niches and Buruli ulcer pathogenesis. PLoS Pathogens 3:e62.​

Other Selected Publications

Kaur, D., Berg, S., Dinadayala, P., Gicquel, B., Chatterjee, D., McNeil, M.R., Vissa, V., Crick, D.C., Jackson, M., and Brennan, P.J. (2006) Biosynthesis of mycobacterial lipoarabinomannan: Role of a branching mannosyltransferase. Proc. Natl. Acad. Sci. USA 103: 13664-13669.

Sulzenbacher, G., Canaan, S., Bordat, Y., Neyrolles, O., Stadthagen, G., Roig-Zamboni, V., Rauzier, J., Maurin, D., Laval, F., Daffé, M., Cambillau, C., Gicquel, B., Bourne, Y., and Jackson, M. (2006) LppX is a lipoprotein required for the translocation of phthiocerol dimycocerosates to the surface of Mycobacterium tuberculosis. EMBO J. 25 : 1436-1444.

Gonzalo Asensio, J., Maia, C., Ferrer, N.L., Barilone, N., Laval, F., Soto, C., Winter, N., Daffé, M., Gicquel, B., Martin, C., and Jackson, M. (2006) The virulence-associated two-component PhoP-PhoR system controls the biosynthesis of polyketide-derived lipids in Mycobacterium tuberculosis. J. Biol. Chem. 281: 1313-1316.

Rousseau, C., Winter, N., Pivert, E., Bordat, Y., Neyrolles, O., Avé, P., Huerre, M., Gicquel, B. and Jackson, M. (2004) Production of phthiocerol dimycocerosates protects Mycobacterium tuberculosis from the cidal activity of reactive nitrogen intermediates produced by macrophages and modulates the early immune response to infection. Cell. Microbiol., 6, 277-287.

Korduláková, J., Gilleron, M., Puzo, G., Brennan, P. J., Gicquel, B., Mikusová, K. and Jackson, M. (2003) Identification of the required acyltransferase step in the biosynthesis of the phosphatidylinositol mannosides of Mycobacterium species. J. Biol. Chem., 278, 36285-36295.

Korduláková, J., M. Gilleron, K. Mikusová, G. Puzo, P. J. Brennan, B. Gicquel, and M. Jackson (2002) Definition of the first mannosylation step in phosphatidylinositol synthesis: PimA is essential for growth of mycobacteria. J. Biol. Chem. 277, 31335-31344.

Raynaud, C., C. Guilhot, J. Rauzier, Y. Bordat, V. Pelicic, R. Manganelli, I. Smith, B. Gicquel, and M. Jackson (2002) Phospholipases C are involved in the virulence of Mycobacterium tuberculosis. Mol. Microbiol. 45, 203-217.

Jackson, M., Crick, D. C. and P. J. Brennan (2000) Phosphatidylinositol is an essential phospholipid of mycobacteria. J. Biol. Chem., 275, 30092-30099.

Jackson, M., Phalen, S. W., Lagranderie, M., Ensergueix, D., Chavarot, P., Marchal, G., McMurray, D. N., Gicquel, B. and C. Guilhot (1999) Persistence and protective efficacy of a Mycobacterium tuberculosis auxotroph vaccine. Infect. Immun., 67, 2867-2873.

Jackson, M., Raynaud, C., Lanéelle, M.-A., Guilhot, C., Laurent-Winter, C., Ensergueix, D., Gicquel B. and M. Daffé (1999) Inactivation of the antigen 85C gene profoundly affects the mycolate content and alters the permeability of the Mycobacterium tuberculosis cell envelope. Mol. Microbiol., 31, 1573-1587.

Pelicic, V., Jackson, M., Reyrat, J.-M., Jacobs Jr., W. R., Gicquel, B. and C. Guilhot (1997) Efficient allelic exchange and transposon mutagenesis in Mycobacterium tuberculosis. Proc. Natl. Acad. Sci USA, 94, 10955-10960.​

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