The ORC is fully equipped to run any commercially available absorbance or fluorescence biomarker immunoassay in 96 or 384-well plate format using Molecular Devices SpecraMax 384 plus, microplate absorbance/transmittance reader, as well as a Gemini-XS Fluorometer.
Our lab has extensive experience with the following biomarker assays:
Detection of Cartilage Markers
Alcian Blue: Standardize measurement of 35S labeled proteoglycan complexes.
C1,C2: An assay to standardize the measurement of Types I and II collagen degradation.
CPII: An assay to measure type II collagen carboxy propeptide (C-propeptide).
CS-846: Measurement of Aggrecan Chondroitin Sulfate 846 Epitope.
Eq. Col 2 3/4 (CEQ): An assay to quantify equine specific Type II collagen, which has also been proven to work with canine fluid.
GAG DMMB: An assay for standardized measurement of glycosaminoglycans in biological fluids and/or tissues.
Prolagen-C: Measurement of C-Terminal propeptide Type-I collagen.
Pyd Assay: An assay to standardize measurement of pyridinoline crosslinks in serum and urine.
Pyrilinks-D: To standardize measurement of deoxypyridinoline crosslinks in urine.
TCA: Assay to measure 3H content in media or cartilage digested samples.
YKL-40: Assay for measurement of YKL-40, human cartilage glycoprotein 39, in serum.
Detection of Bone Markers
C1,2C: An assay to standardize measurement of Type I and II collagens (378 assay, MMP1 and MMP13).
Metra™ BAP: Quantification of bone-specific alkaline phosphatase in serum and synovial fluid samples.
Metra™ Osteocalcin EIA: An enzyme immunoassay for the quantification of intact (de novo) osteocalcin.
Serum Cross Laps® (CTX): Assay for the quantification of degradation products of C-terminal telopeptides of Type-I collagen in serum and plasma.
HIL-1ra: To standardize the measurement of interleukin 1 receptor antagonist concentrations in cell culture supernatant, serum and plasma.
IGF: To standardize the measurement of Insulin-like Growth Factor in Serum, Cell culture and plasma.
TGF-β: An assay to quantify measurement of Transforming Growth Factor-beta in serum, cell culture supernatant, plasma and urine.
TNF-alpha: An assay to quantify levels of Tumor Necrosis Factor-alpha in serum, plasma, synovial fluid and cell culture supernatant
IL-10: An assay to quantify levels of Interleukin-10 in serum, plasma and cell culture supernatant.
PDGF-BB: An assay to quantify levels of Platelet-Derived Growth Factor-BB subunit in serum, plasma and cell culture supernatant.
PGE2: An assay to quantify levels of Prostaglandin E2 in serum, plasma, synovial fluid, cell culture supernatant and urine.
Pre-assay sample processing including: papain, hyaluronidase and collogenase digestion, as well as chromatography extraction of synovial fluid and tissues
Western, Southern, and Northern Blotting
Many other assays are available. Please inquire.
Displacement control testing for compressive, tension and shear material properties
Tissue explants or cell-seeded scaffolds
Light to moderate load cells are suitable for testing small tissue explants or cell-seeded scaffolds
Evaluation of metabolic activity in living tissues
- Radiolabel protocols available
GeneChip® Microarray Analysis
- Complete Affymetrix GeneChip® 3000 scanner, fluidics 450 and hybridization system
Real Time PCR Analysis
- ABI Prism® 7000 Sequence Detection System
- Optimization of PCR Primers
- DNA synthesis from RNA
- RNA from cells, tissue or whole blood
- Primer and probe design
- Gel extraction and purification
- Purification of plasmid DNA
- PCR amplification
Isolation of Synoviocytes, Chondrocytes, and Tenocytes
- Cell culture expansion of freshly collected cells
Culturing of Mesenchymal Stem Cells (bone-marrow derived or fat-derived)
- Cell culture expansion of bone-marrow derived or adipose-derived cells, including three-dimensional culturing for clinical use
Adenoviral Vector Construction and Cell Transfection
- The development of adenoviral vectors for the delivery of genes into cells
Decalcified tissue histology
Paraffin and frozen Sectioning and staining of paraffin embedded samples